Friday, April 11, 2008

Help needed for ELISpot assay

One of my experiments involves performing quality check of the spots developed on a 96-well plate for an ELISpot assay. Akin to spotting the differences between two pictures.

Can anyone share their experience of how to get rid of the debris that was seen on the plate? I did filtering of the dilution and washing buffers. So, I am wondering what could attribute to it and at which step of the 3-day experiment?

2 comments:

Anonymous said...

I find that liquid Cascade does an excellent job of removing debris from my plates ... and the glasses come out of the dishwasher absolutely spotless! Hope this tip works for you.

Paul said...

Thank you.