Recounting back in time

It has been almost one month since I posted. During this period, I was extremely busy with experiments and preparing slides for update of National Institutes of Health (NIH) grant project, and culturing cells.

As my project involves screening different viral peptides for the entire lab, I had screened more than 600 peptides for binding to Major Histocompatibility Complex molecules (with help from fellow colleagues) in this short six months. The large data generated had to be processed and presented with results from prediction models.

Before the presentation on Oct 20, 2006, the atmosphere in the lab was tense. We (Yang Kun, Milton, Eduardo and I) were busy preparing slides. Dry runs were performed to improve our slides.

A farewell party at the Johns Hopkins Faculty Club was organized for Yang Kun, who would be going back to China on Oct 30. I will miss a good colleague and friend whom I had come to cherish in this short time. We also celebrated Tom's birthday on Oct 19 where he turned 79. I admire him for being so alert and active at his age!

On 'D-Day', I was the first to present where my voice quivered at the beginning. Eventually, I got over it as Linda (from NIH) was pleasant. Lunch that day was exorbitant and delicious, consisting of sandwiches, cheese and salad. We even had a lot left over for the next day.

Life on Oct 21 was back to normalcy of conducting experiments.

How to tie a Windsor knot as you see it (not mirror images)

Most instructions for tying a full Windsor knot have you facing the mirror. It can be difficult to follow when you have to visualise your left from right. Thus, I have created a set of instructions whereby you are tying a Windsor knot as you see it, from head down.

Don't worry about the length of the wide end of the tie as our body shapes and sizes are different. Some have big chest or belly, short or long torso, narrow or broad neck etc. There are some who have ties reaching way below their belts or hanging high above. The ideal length of a tie is when the tip of the wide end touches above the belt or covering it, but not exceeding beyond the belt when you are standing straight. Practise allows you to judge how much slack to give for the wide end.

The Windsor knot projects confidence. Tie this knot during important occasions such as job interviews, presentations etc.

For easy visualization of tying the Windsor knot, the top is striped green and the underside is blue.

1. Place the narrow end of a tie on your left and the wide end on your right. (Tying the knot involves the wide end going around the narrow end i.e. the narrow end is fixed.)

2. Pinch the narrow end of the tie where you want the wide end to cross over and flip the wide end under the loop and bring it up to your left.

3. Pull the wide end to the right, behind the loop and go over the loop again. You should see a triangular knot upon tightening.

4. Bring the wide end to the left over the triangular knot, go under the loop and bring it up. Pass the wide end through the knot in front.

5. Adjust the size of the knot with both hands by tightening the knot.

6. Draw up the tie with one hand holding the knot while the other pulls the narrow end down.

7. For quick release of the Windsor knot, pull at the left loop above the knot.

Taking care of myself

Being sick is bad but to be sick in a foreign place is worse. You have to perform daily chores such aspreparing your meals even though you are tired and feeling unwell.

It started with throat irritation on Wednesday. Sucking lozenges did not help, so I prepared sour plum with honey. My sored throat recovered but I started to cough.

After self medicating by buying cough syrup from the pharmacy, I was able to control my coughing. But with the bad weather due to Hurricane Ernesto, my nose started to run and now I have blocked nose.

A good thing this Monday is Labor Day in USA. An extra day of rest for me to recuperate and recover.